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- W2349203588 abstract "The effect of goat spermatozoa capacitation induced by heparin or calcium ionophore A23187 in vitro on the transfection exogenous DNA into spermatozoa was evaluated in our study.Seminal ejaculates collected artificially from healthy adult bucks were pooled together and the washed sperm were incubated in vitro for 10 mins in the capacitation medium with 0.4 μmol/L calcium ionophore A23187(IA) or 90 mins in the medium with 10 mg/L heparin(HE),of modified Tyrode's solution at 38.5℃with sperm concentrations of 10-20 million/mL.The activated sperm motility and acrosome reaction were assessed.The induced sperm were incubated with exogenous DIG-labeled linear DNA for 60 mins.The transfection rate of sperm was observed by the in situ hybridization methods.The ultrastructure of activated spermatozoa were tested by transmission electron microscope(TEM),and the integrity of sperm plasma membrane was evaluated with a combination of fluorescent probes-carboxifluorescein diacetate and propidium iodide(PI).The results showed that sperm capacitation significantly reduced the efficiency of spermatozoa in picking up exogenous DNA(P0.01).The efficiency of IA,HE and untreated sperm were(17.41±4.69)%,(15.00±4.36)% and(32.97±4.58)%,respectively.The efficiency of subsequently internalize the DNA into sperm nuclei were(12.09±2.50)%,(8.93±1.79)% and(25.52±2.57)%,respectively.And the spermatozoa capacitation significantly reduced the integrity of sperm plasma membrane(P0.01).It would suggest that DNA uptake by the mammalian spermatozoa is a very specific and high regulated phenomenon." @default.
- W2349203588 created "2016-06-24" @default.
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- W2349203588 date "2009-01-01" @default.
- W2349203588 modified "2023-09-25" @default.
- W2349203588 title "Effect of goat sperm capacitation induced by heparin or calcium ionophore A23187 in vitro on the transfection exogenous DNA into spermatozoa" @default.
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