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- W2349204072 abstract "The paper is the establishment of an ELISA for measurement of vitellin in Chrysopa septempunctata. The vitellin of Chrysopa septempunctata was extracted with cold distilled water. A polyclonal antibody against vitellin was prepared in male rabbits and then it was made the specific antibody for vitellin by absorbtion with male worm homogenate. As the result, specific antibody could be obtained which was anti-vitellin. Through purification, HRP was used to mark antibody, then ELISA was established to detect vitellin content in the Chrysopa septempunctata fat body, hemolymph and ovary. The result implied that the vitellogenin was synthesized in fat body at the beginning of the fourth day after eclosion. The content of vitellogenin in fat body and haemolymph increased on the fourth day after eclosion quickly and reached the peak stage on the seventh day in adult period. The vitellin in ovary could be detected on the fourth day after eclosion. Then the content of vitellin increased quickly and reached the peak stage on the seventh day in adult period. SDS-PAGE analysis showed that the vitellin was composed of two subunits, one large 165 kD and one small 46 kD." @default.
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- W2349204072 date "2004-01-01" @default.
- W2349204072 modified "2023-09-22" @default.
- W2349204072 title "Dynamic Analysis on the Vitellogenesis of Chrysopa septempunctata" @default.
- W2349204072 hasPublicationYear "2004" @default.
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