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- W2349204789 abstract "Objective To develop a sensitive, specific, and economic HCMVpp65 antigenemia assay for clinical application. Methods We took human embryonic lung fibroblast(HELF) infected with HCMV AD169 as positive control, and selected appropriate reagents to combine a new kit. Peripheral polymorphonuclear leukocytes (PMNLs) were isolated by sedimentation with a 6% dextran solution in 0.9% saline. The fixation was using 4% formalin. Slides were stained by an indirect immunoperoxidase staining with a commercially available mixture of monoclonal antibodies C 2/C 6. Then compared with a commercial indirect immunofluorescence assay kit. And then we carried a prospective, paralled, and blind study on 100 blood samples in order to compare our result with a fluorescent quantitative PCR assay. Results Our kit could successfully detect positive PMNLs for pp65. Its sensitivity corresponded with HELFs infected by 10 2 pfu/ml AD169 for 24 hours or 3 5 positive cells/2×10 5 PMNLs. Dextran with high molecular weight could rapidly and efficiently extract PMNLs from peripheral blood. Cytospins fixed with 4% formalin could be stored (-20 °C ) for a long time with stability of pp65 antigen. Conclusions The optimized kit in our laboratory had no statistical difference in the detection of pp65 antigenemia comparing with congeneric commercial kits. And its result coincided with that of FQ PCR. But our kit is simpler, less expensive, and more applicable for clinical applications." @default.
- W2349204789 created "2016-06-24" @default.
- W2349204789 creator A5085508139 @default.
- W2349204789 date "2004-01-01" @default.
- W2349204789 modified "2023-09-23" @default.
- W2349204789 title "Development and optimization of a laboratory assay for the human cytomegalovirus pp65 antigenemia and its preliminary clinical application" @default.
- W2349204789 hasPublicationYear "2004" @default.
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