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- W2349274966 abstract "Objective To study the binding properties of radioiodinated peptide neurotensin analog ~(125)I-NT_2 through its binding with HT29 cells in vitro.Methods NT_2(Lys-Arg-Pro-Tyr-Tle-Leu)was la- beled with Na~(125)I by Ch-T,purified by Sep-Pak column and assessed by high performance liquid chromatog- raphy(HPLC).The incubation time,cell concentration and temperature for ~(125)I-NT_2 binding to HT29 cells were carried out in order to obtain optimal labeling condition.Saturable binding between ~(125)I-NT_2 and HT29 cells was also performed in order to investigate the binding affinity.Results ①The labeling rate of ~(125)I- NT_2 was 76%.The radiochemical purity was98%.The specific activity was 9.71×10~(12) Bq/mmol.② The optimal condition of binding:cell number,temperature and time of incubation were 10~6,25℃ and 1 h respectively.The K_d and B_(max) were(0.745±0.418)nmol/L and(0.103±0.060)pmol/10~6 cell.Con- clusion ~(125)I-NT_2 has high affinity of binding to HT29 cells and it may be a promising targeting agent for the imaging of colon adenocarcinoma." @default.
- W2349274966 created "2016-06-24" @default.
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- W2349274966 date "2006-01-01" @default.
- W2349274966 modified "2023-09-28" @default.
- W2349274966 title "In vitro binding characteristics of ~(124)I labeled neurotensin analogue NT_2 to human colonic adenocarcinoma cell line HT29" @default.
- W2349274966 hasPublicationYear "2006" @default.
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