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- W2349293775 abstract "The adaptor of the pRevTet-On and pIRES2-EGFP was designed,by a series of digestion and ligation,to obtain a new recombinant retroviral vector pRevIRES2-EGFP which includes the EGFP gene and MCS.Meanwhile,the pBV220-IL-2 and pEGFP-C1 are recombined as a new vector pEGFP-C1-IL-2 which includes the IL-2 gene by the same method.Then pEGFP-C1-IL-2 is digested to recovery the fragment of IL-2 gene by agarose gel electrophoresis and ligated it to the MCS of pRevIRES2-EGFP.Amplifying the plasmid in the E.coli DH5α we obtain the recombinant vector pRevIRES2-EGFP-IL-2.The analytic results showed that the retroviral expression vector pRevIRES2-EGFP-IL-2 was correct completely." @default.
- W2349293775 created "2016-06-24" @default.
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- W2349293775 date "2005-01-01" @default.
- W2349293775 modified "2023-09-28" @default.
- W2349293775 title "Construction of Retroviral Vectors Co-expressing the IL-2 and EGFP" @default.
- W2349293775 hasPublicationYear "2005" @default.
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