Matches in SemOpenAlex for { <https://semopenalex.org/work/W2349312179> ?p ?o ?g. }
Showing items 1 to 63 of
63
with 100 items per page.
- W2349312179 abstract "Objective To establish a rapid multiplex PCR assay to identify and differentiate the five main pathotypes of diarrheogenic Escherichia coli and Shigella spp. Methods The virulence genes stx1,stx2,eaeA,lt,stIb,aggR,ipaH as the target genes and 16S RNA gene rrs as an internal control gene were grouped into a multiplex PCR reaction system. By optimizing the multiplex PCR primers,reaction conditions and cycle parameters,the multiplex PCR can identify and differentiate the five main pathotypes of diarrheogenic Escherichia coli and Shigella spp rapidly and accurately. ResultsThe established multiplex PCR assay was applied in this study to identify 87 strains of Escherichia coli and Shigella spp. The results showed that the genetypes were 100% consistent with those of single PCR amplification. Conclusion The multiplex PCR assay in this study can be used not only to identify the five main pathotypes of diarrheogenic Escherichia coli and Shigella spp in a single reaction,but also to evaluate the efficiency. This assay is an effective supplement to other molecular methods in the identification of the five main pathotypes of diarrheogenic Escherichia coli and Shigella spp." @default.
- W2349312179 created "2016-06-24" @default.
- W2349312179 creator A5071010006 @default.
- W2349312179 date "2011-01-01" @default.
- W2349312179 modified "2023-09-26" @default.
- W2349312179 title "Multiplex PCR for identification of diarrheogenic Escherichia coli and Shigella spp" @default.
- W2349312179 hasPublicationYear "2011" @default.
- W2349312179 type Work @default.
- W2349312179 sameAs 2349312179 @default.
- W2349312179 citedByCount "0" @default.
- W2349312179 crossrefType "journal-article" @default.
- W2349312179 hasAuthorship W2349312179A5071010006 @default.
- W2349312179 hasConcept C104317684 @default.
- W2349312179 hasConcept C2776985332 @default.
- W2349312179 hasConcept C2776986154 @default.
- W2349312179 hasConcept C2778411228 @default.
- W2349312179 hasConcept C2781001907 @default.
- W2349312179 hasConcept C2781188995 @default.
- W2349312179 hasConcept C49105822 @default.
- W2349312179 hasConcept C54355233 @default.
- W2349312179 hasConcept C547475151 @default.
- W2349312179 hasConcept C86803240 @default.
- W2349312179 hasConcept C89423630 @default.
- W2349312179 hasConcept C90583042 @default.
- W2349312179 hasConceptScore W2349312179C104317684 @default.
- W2349312179 hasConceptScore W2349312179C2776985332 @default.
- W2349312179 hasConceptScore W2349312179C2776986154 @default.
- W2349312179 hasConceptScore W2349312179C2778411228 @default.
- W2349312179 hasConceptScore W2349312179C2781001907 @default.
- W2349312179 hasConceptScore W2349312179C2781188995 @default.
- W2349312179 hasConceptScore W2349312179C49105822 @default.
- W2349312179 hasConceptScore W2349312179C54355233 @default.
- W2349312179 hasConceptScore W2349312179C547475151 @default.
- W2349312179 hasConceptScore W2349312179C86803240 @default.
- W2349312179 hasConceptScore W2349312179C89423630 @default.
- W2349312179 hasConceptScore W2349312179C90583042 @default.
- W2349312179 hasLocation W23493121791 @default.
- W2349312179 hasOpenAccess W2349312179 @default.
- W2349312179 hasPrimaryLocation W23493121791 @default.
- W2349312179 hasRelatedWork W126204657 @default.
- W2349312179 hasRelatedWork W1604447352 @default.
- W2349312179 hasRelatedWork W1920477678 @default.
- W2349312179 hasRelatedWork W2061420678 @default.
- W2349312179 hasRelatedWork W2112667766 @default.
- W2349312179 hasRelatedWork W2117634096 @default.
- W2349312179 hasRelatedWork W2129305135 @default.
- W2349312179 hasRelatedWork W2301099699 @default.
- W2349312179 hasRelatedWork W2363796151 @default.
- W2349312179 hasRelatedWork W2365776297 @default.
- W2349312179 hasRelatedWork W2368140824 @default.
- W2349312179 hasRelatedWork W2371435784 @default.
- W2349312179 hasRelatedWork W2375780942 @default.
- W2349312179 hasRelatedWork W2378229653 @default.
- W2349312179 hasRelatedWork W2378335104 @default.
- W2349312179 hasRelatedWork W2379534692 @default.
- W2349312179 hasRelatedWork W2382597736 @default.
- W2349312179 hasRelatedWork W2394168063 @default.
- W2349312179 hasRelatedWork W2412385802 @default.
- W2349312179 hasRelatedWork W2986036840 @default.
- W2349312179 isParatext "false" @default.
- W2349312179 isRetracted "false" @default.
- W2349312179 magId "2349312179" @default.
- W2349312179 workType "article" @default.