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- W2349489021 abstract "Microorganisms capable of producing biosurfactants are isolated by enrichment culture, blue agar plate and determination of the surface tension of fermentation liquor. Eight strains with high surface activity are isolated from the samples of wastewater and oil sludge (collected from Nanjing Refinery) and two of them (BS-03 and BS-01) are for further study. Through primary identification both strain BS-03 and strain BS-01 belong to Pseudonomas. The biosurfactant produced by strain BS-03 can lower the surface tension of fermentation liquor from 56.8 mN/m to 25.6 mN/m and the extract that yields CMC value of 326 mg/L while that of strain BS-01 is 27.4 mN/m and 58 mg/L separatedly. Characterization of the products of two strain susing electrospray ionizationmass spectrometry reveals that monorhamnolipid (RhaC_(10)C_(10)) is predominant in extract of strain BS-03, while dihamnolipids (Rha_(2)C_(10) and Rha_(2)C_(10)C_(10)) are predominant in that of strain BS-01. Emulsification activity of fermentation liquor of strain BS-03 is greater than 70% using all the hydrocarbons tested, including benzene, hexane, octadecane, diesel and crude oil. But strain BS-01 only emulsifies these hydrocarbons below 60%." @default.
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- W2349489021 date "2005-01-01" @default.
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- W2349489021 title "Isolation of microbe producing biosurfactants by blue agar plate" @default.
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