Matches in SemOpenAlex for { <https://semopenalex.org/work/W2349544107> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W2349544107 abstract "Sea cucumbers(Stichopus japonicus) belonging to the phylum Echinodermata are traditional tonic consumed by Chinese and Japanese.The major edible parts of sea cucumber are the body wall mainly consisting of collagen and mucopolysaccharides.More than 21 types of collagen have so far been identified in various tissues and their roles have been investigated.In invertebrate,at least 2 types of collagen,type Ⅰ and type Ⅴ are found to exist.There is little information about the collagen of sea cucumber except for few reports on S.japonicus and Cucumaria frondosa,respectively.However,results of the former findings were not consistent with that of the latter about the molecular composition of the sea cucumber collagen.In this paper,the intact collagens were isolated from the body wall of S.japonicus by a new method and their physical and chemical characterization were studied.The results showed that pepsin-solubilized collagens(PSC) without telopeptide could be prepared at low temperature.Through the ultraviolet scanning spectra from 190 to 400 nm,the PSC presented maximum absorption at 220 nm which was consistent with that of type Ⅰcollagen.The compositional features of the amino acid were with the high contents of glycine(329 residues/1000) and hydroxyproline(66 residues/1000),but with low contents of tyrosine,phenylalanine and histidine.No cysteine was detected.The ratio of hydroxyproline to proline was 0.69.It was concluded that PSC was typical type Ⅰcollagen.This was the first report about the thermal stability temperature(T_s) of sea cucumber collagen which was 57 ℃ as measured by DSC,about 5 ℃ lower than that of type Ⅰcollagen of calf.The content of total polysaccharide determined with phenol-sulfuric acid method was 0.61% and the glycosaminoglycan measured with cationic dye of methylene blue was 0.48%.The collagen could be separated from glycosaminoglycan by DEAE-52.Based on SDS polyacrylamide gel electrophoresis(SDS-PAGE),the purities of PSC were higher.Judging from their electrophoretic patterns,PSC contained β,α and a few γ chains,while α chain resembled α_1 chain of type-Ⅰcollagen of vertebrate,however,the presence of(α_1)_3 trimers was evident." @default.
- W2349544107 created "2016-06-24" @default.
- W2349544107 creator A5090024150 @default.
- W2349544107 date "2006-01-01" @default.
- W2349544107 modified "2023-09-26" @default.
- W2349544107 title "Extraction and the physical and chemical characterization of collagen in Stichopus japonicus" @default.
- W2349544107 hasPublicationYear "2006" @default.
- W2349544107 type Work @default.
- W2349544107 sameAs 2349544107 @default.
- W2349544107 citedByCount "1" @default.
- W2349544107 countsByYear W23495441072014 @default.
- W2349544107 crossrefType "journal-article" @default.
- W2349544107 hasAuthorship W2349544107A5090024150 @default.
- W2349544107 hasConcept C134018914 @default.
- W2349544107 hasConcept C18903297 @default.
- W2349544107 hasConcept C2776093933 @default.
- W2349544107 hasConcept C2776760561 @default.
- W2349544107 hasConcept C2778460671 @default.
- W2349544107 hasConcept C2779797417 @default.
- W2349544107 hasConcept C2779815552 @default.
- W2349544107 hasConcept C2780644872 @default.
- W2349544107 hasConcept C515207424 @default.
- W2349544107 hasConcept C55493867 @default.
- W2349544107 hasConcept C86803240 @default.
- W2349544107 hasConceptScore W2349544107C134018914 @default.
- W2349544107 hasConceptScore W2349544107C18903297 @default.
- W2349544107 hasConceptScore W2349544107C2776093933 @default.
- W2349544107 hasConceptScore W2349544107C2776760561 @default.
- W2349544107 hasConceptScore W2349544107C2778460671 @default.
- W2349544107 hasConceptScore W2349544107C2779797417 @default.
- W2349544107 hasConceptScore W2349544107C2779815552 @default.
- W2349544107 hasConceptScore W2349544107C2780644872 @default.
- W2349544107 hasConceptScore W2349544107C515207424 @default.
- W2349544107 hasConceptScore W2349544107C55493867 @default.
- W2349544107 hasConceptScore W2349544107C86803240 @default.
- W2349544107 hasLocation W23495441071 @default.
- W2349544107 hasOpenAccess W2349544107 @default.
- W2349544107 hasPrimaryLocation W23495441071 @default.
- W2349544107 hasRelatedWork W1935626829 @default.
- W2349544107 hasRelatedWork W1974356154 @default.
- W2349544107 hasRelatedWork W1975819226 @default.
- W2349544107 hasRelatedWork W1985073578 @default.
- W2349544107 hasRelatedWork W1986613552 @default.
- W2349544107 hasRelatedWork W1991559011 @default.
- W2349544107 hasRelatedWork W1991726724 @default.
- W2349544107 hasRelatedWork W1995803271 @default.
- W2349544107 hasRelatedWork W1998708819 @default.
- W2349544107 hasRelatedWork W2004212722 @default.
- W2349544107 hasRelatedWork W2019791304 @default.
- W2349544107 hasRelatedWork W2033818912 @default.
- W2349544107 hasRelatedWork W2046796228 @default.
- W2349544107 hasRelatedWork W2062954964 @default.
- W2349544107 hasRelatedWork W2073068995 @default.
- W2349544107 hasRelatedWork W2198858055 @default.
- W2349544107 hasRelatedWork W2366937802 @default.
- W2349544107 hasRelatedWork W2794609689 @default.
- W2349544107 hasRelatedWork W2805645197 @default.
- W2349544107 hasRelatedWork W2184990020 @default.
- W2349544107 isParatext "false" @default.
- W2349544107 isRetracted "false" @default.
- W2349544107 magId "2349544107" @default.
- W2349544107 workType "article" @default.