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- W2349654488 abstract "In order to verify the feasibility of PFGE technology on finding out the different genes associated with Cr( VI)removal between ATCC 9372 and Ua strains,the strains were cultured by LB medium which contains 0,20,40,60,80mg·L-1Cr( VI),OD600 and removal rates were measured at 0,12,24,36,48,60 h. PFGE technology was used to study the differences of DNA between two strains which have been enzyme. The feasibility of PFGE technology was verified by PCR and analysis of restriction sites. GM technology was used to test the function of different genes on removing Cr( VI). Results showed that the growth conditions and removal rates of ATCC 9372 and Ua strains reached the maximum at 48 h,ATCC 9372 strain grew better than Ua strain,and its removal rate reached 95% in 40 mg·L-1Cr( VI) solution as well as the removal rate of Ua strain just reached 52. 31%. There is a 3Kb different fragment which had been digested by EcoRI-Hind III between ATCC9372 and Ua strains was showed by the results of PFGE technology.Yth A and Yti B genes were contained in the different fragment. The feasibility of PFGE to explore difference among DNA is credible which was tested by PCR and the analysis of restriction sites. The removal rate of genetically modified strain ATCC 9372-Yti B declined 12% reflected that gene Yti B can inhibit the function of removing Cr( VI). Our study provide a new approach of searching different genes theoretically,and the approach need further improvement." @default.
- W2349654488 created "2016-06-24" @default.
- W2349654488 creator A5021043646 @default.
- W2349654488 date "2015-01-01" @default.
- W2349654488 modified "2023-09-27" @default.
- W2349654488 title "Analyzation on Genetic Variations Associated With Cr(VI) Removal Between two Bacillus atrophaeus Strains Applied PFGE Technology" @default.
- W2349654488 hasPublicationYear "2015" @default.
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