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- W2349657784 abstract "Objective:To establish a method for isolating,purifying and culture cardiomyocytes from neonatal BALB/c mice.Methods:Myocardial tissues from neonatal BALB/c mice born within 1 to 3 day were digested with 0.125% trypsin and 0.1% collagenaseⅠ.The cardiomyocytes were collected in DMEM medium,which contains 15% fetal bovine serum.Purification of cardiomyocytes was done by differential adhesion and the supplement of 5-bromodeoxyuridine.Cardiomyocytes were identified by immunohistochemistry staining of α-actin and we stained Cardiomyocytes with trypan blue.Results:After 24 hours some single cardiomyocytes began to beat spontaneously.After 48 hours,cardiomyocytes got together and beat synchronously.Cells used in experiments were confirmed as cardiomyocyte and 95 percent were alive.Conclusion:High quantity and survival cardiomyocytes of BALB/c mice can be effectively and reliably cultured by this method." @default.
- W2349657784 created "2016-06-24" @default.
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- W2349657784 date "2011-01-01" @default.
- W2349657784 modified "2023-09-25" @default.
- W2349657784 title "The primary culture of cardiomyocytes from neonatal BALB/c mice" @default.
- W2349657784 hasPublicationYear "2011" @default.
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