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- W2349672223 abstract "Aim To establish a model of SNP-induced dorsal root ganglion(DRG) neuron injury based on the method of purifying DRG neuron primary cultures from the neonatal rats.Method DRG were taken out from the intervertebral foramen of neonatal rats and separated into single-celled suspension liquid using collagenase-Dispase,5-FUDR were added to purify the cells,MEM-F12 medium were used to cultivate the cells and NSE immunohistochemical method were applied to identify the DRG neurons.DRG neurons were treated with sodium nitroprusside (SNP) at the eighth day with different concentrations (500,50,5μmol·L-1).Cells were measured by MTT assay and trypan blue viability test.Results Isolated and cultured DRG neurons survived and grew nicely more than two weeks in the feeding medium,and the purified rate of DRG neurons could be above 90%.The increase of DRG neuron death rate was proportional to the rise of the concentration of the SNP.Conclusion This method is of high stability and efficiency.The neuronal population can be better purified with 5-FUDR.The DRG injury neuron model with SNP preparation could be used as an ideal in vitro model in the relevant experimental studies." @default.
- W2349672223 created "2016-06-24" @default.
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- W2349672223 date "2010-01-01" @default.
- W2349672223 modified "2023-09-28" @default.
- W2349672223 title "Establishment of a dorsal root ganglion neurons injury model induced by SNP" @default.
- W2349672223 hasPublicationYear "2010" @default.
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