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- W2349701178 abstract "To establish a high performance liquid chromatographic(HPLC) method for determination of docetaxel in hepatocellular cancer cells.Docetaxel was extracted from hepatocellular cancer cells by a Bond-elut C18 solid phase extraction(SPE)column,and separated on a Agilent TC-C18(5 μm,4.6 mm×150 mm) column by using mobile phase consisted of ammonium acetate buffer(0.02 mol/L,pH 5.0)-acetonitrile(50∶50,by volume) with a flow rate of 1 mL/min at detection wavelength of 230 nm.The result indicated that the calibration of docetaxel was linear in the range of 0.05-2.25 mg/L with correlation coefficient of 0.999 3.The detection limit for docetaxel was 0.03 mg/L.The extraction recoveries of docetaxel form forin cells sample ranged from 93% to 95%.The within-day RSDs(n=5) and the between-day RSDs(n=5) were both not more than 6.9%.The method was convenient,accurate and sensitive,and could be applied in the determination of low level of docetaxel in biological samples." @default.
- W2349701178 created "2016-06-24" @default.
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- W2349701178 date "2010-01-01" @default.
- W2349701178 modified "2023-09-28" @default.
- W2349701178 title "Solid Phase Extracted/HPLC Method for Determination of Docetaxel in Hepatocellular Cancer Cells" @default.
- W2349701178 hasPublicationYear "2010" @default.
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