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- W2349709928 abstract "A surface plasmon resonance(SPR)biosensor has been established for rapid detection of Escherichia coli.An activation step performed on CM5 chips was carried using EDC/NHS.The biotin-labeled secondard antibody(goat anti-rabbit IgG)was immobilised onto CM5 chip modified with streptavidin via the biotin-streptavidin interaction.Then,rabbit anti-E.coli ATCC25922 polyclonal antibody(primary antibody)was immobilized on sensor chip by antibody-antigen recognition.The detectable signal was amplified by the amplification of primary and secondary antibodies and the streptavidin-biotin amplification strategy.All the SPR experiments were performed used a Biacore 3000 and CM5 chips.Regeneration was achieved using NaOH in order to detect several samples.The change of RU was linearly correlated with the concentration of E.coli ATCC25922(r=0.981 5).This method exhibited high performance with a dynamic range of 1.5×102-1.5×107 CFU/mL,and a detection limit was 1.5×102 CFU/mL.This method has exhibited great potential in bacteriological testing." @default.
- W2349709928 created "2016-06-24" @default.
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- W2349709928 date "2013-01-01" @default.
- W2349709928 modified "2023-09-23" @default.
- W2349709928 title "Streptavidin(SA)-Biotin Amplification Strategy Based SPR Sensor forRapid Detection of Escherichia Coli" @default.
- W2349709928 hasPublicationYear "2013" @default.
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