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- W2349733236 abstract "Trichosanthin (TCS),an effective aborticide injures specifically the conceptus without defects on maternal tissues.It can induce mid-term abor-tion in some laboratory animals.The present study deals with the TCS distribution in various tissues of the pregnant mice after different injec-tion times.We adopted horseradish peroxidase immunoenzyme cytochemi-cal methods,especially PAP complex techique to demonstrate the TCS dis-tribution and selected veronal buffer as washing fluid to prevent diffusion of TCS on sections.After failures in the fixation of TCS in tissues with conventional fixatives,a new fixative PPAA was designed for the pre-cise localization of TCS.It was found from the labelling results that TCS appeared not only in placental cells,but also in all maternal tissues as well as in foetal cells.It was presentin liver cells (fig.2) and cardiac muscle cells (fig.4).It was found in pro-minent amount in the proximal con-voluted tubules of the renal cortex (fig.6),and less so in labyrinth,giant trophoblastic cells and decidual cells (figs.10,11,12,and 13).It also appeared in cerebral neurones (fig.7).Therefore neither blood-brain barrier nor placental barrier was found by this method.The positive reactions of related cell types appeared soon after injection of the drug,and lasted for a rather short time.Except in a few cases,TCS was found to be absent from the nuclei and was generally localized in the cytoplasm.The amount of TCS deposition in various types of cells is perhaps related with the physiological status of the cell,and not correlated to the injury drug has caused." @default.
- W2349733236 created "2016-06-24" @default.
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- W2349733236 date "1982-01-01" @default.
- W2349733236 modified "2023-09-25" @default.
- W2349733236 title "LOCALIZATION OF TRICHOSANTHIN IN TISSUES OF PREGNANT MOUSE AS SHOWN BY IMMUNO-CYTO CHEMICAL TECHNIQUES" @default.
- W2349733236 hasPublicationYear "1982" @default.
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