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- W2349761786 abstract "In order to expound the secretion protein changes during yeast aging,the yeast was cultivated in saline and its extracellular protein content and activities of antioxidant enzymes and protease were determined. The effect of long term culture on the senescence of these cells was evaluated. Total extracellular were extracted and separated by two-dimensional electrophoresis,and compared with extracellular protein of normal( YNB) culture. Then the different points between them were identified by MALDI-TOF-MS. Under the condition of continuous aging the intracellular antioxidant enzyme activity was reduced,while the activity of protease and the amount extracellular protein increased at the same time. The results from MALDI-TOF-MS showed that extracellular protein contains some intracellular protein fragments. It might be related to the increasing activities of protease in cells. Intracellular useless proteins were hydrolyzed into pieces to extracellular environment." @default.
- W2349761786 created "2016-06-24" @default.
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- W2349761786 date "2015-01-01" @default.
- W2349761786 modified "2023-09-23" @default.
- W2349761786 title "Changes of extracellular proteins of Saccharomyces cerevisiae during continuous aging" @default.
- W2349761786 hasPublicationYear "2015" @default.
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