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- W2349762279 abstract "Objective To establish a reliable SYBR Green real-time PCR method for detecting the effect of IFN-γ on the expression pattern of versican isoforms in rat neural stem cell(NSCs).Methods The NSCs were isolated from embryonic rat and cultured in growth medium supplement with EGF and bFGF in vitro.For cytokine stimulation,the cells were incubated with IFN-γ for 48 hours,then collected for RNA extraction and reverse transcription.The primers were designed for different versican isoforms based on their gene sequence.After optimization of PCR parameter,real-time PCR was carried out on an ABI7900 PCR Detection System with fluorescence dye SYBR Green.The relative quantification analysis was performed with comparative threshold cycle(△△Ct)method.Results Melting curve analysis and agarose gel electrophoresis confirmed the specificity of the amplification products.Exposure to 100U/ml IFN-γ could upregulated the expression of versican V2 by 2.9 folds(P0.01),but had no effect on versican V1.Conclusion The developed real-time PCR assay for detecting the expression of versican isoforms,with high specificity and good quality,is suitable to investigate the expression pattern of versican isoforms and its regulation mechanism in development and diseases." @default.
- W2349762279 created "2016-06-24" @default.
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- W2349762279 date "2008-01-01" @default.
- W2349762279 modified "2023-09-24" @default.
- W2349762279 title "Detecting the Regulation of IFN-γ on the Expression of Versican Isoforms in Rat Neural Stem Cells by SYBR Green Real-time PCR" @default.
- W2349762279 hasPublicationYear "2008" @default.
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