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- W2349771010 abstract "Aim: To investigate the transfection efficiency and distribution of oligodeoxynuclotide in human retinal pigment epithelial cells mediated by cationic liposome LipofectamineTM 2000.Methods:Fluorescence FAM labeled oligodeoxynuclotide was introduced into RPE cells by LipofectamineTM 2000.The location of fluorescence in RPE cells at 20 min,1 h,2 h,4 h,6 h,12 h,and 24 h after transfection was observed by fluorescence microscope,transfection efficiency was detected by flow cytometry,and the cytotoxicity was measured by MTT assay.Results: FAM-ODNs complexed with LipofectamineTM 2000 were transfected into cytoplasm and nucleus of RPE cells rapidly,and the highest transfect rate reached(85.85±2.75)% after 4 to 6 h incubation(P0.05),(70.11±5.81)% after 24 h.No cytotoxicity was observed(P0.05).Conclusion: LipofectamineTM 2000 could effectively transfect FAM-ODNs into RPE cells and it is a safe and effective vector for gene therapy." @default.
- W2349771010 created "2016-06-24" @default.
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- W2349771010 date "2008-01-01" @default.
- W2349771010 modified "2023-09-28" @default.
- W2349771010 title "Detection of transfection efficiency and distribution of oligodeoxynuclotide into human retinal pigment epithelial cells mediated by cationic liposome" @default.
- W2349771010 hasPublicationYear "2008" @default.
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