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- W2349780147 abstract "OBJECTIVE To optimize the ploymerase chain reaction and denature gradient gel electrophoresis(PCR-DGGE) technique that was used to analyze the community composition of endophytic fungi for Ligusticum chuanxiong Hort.METHODS The gradient range and electrophoresis time of PCR-DGGE system based on its region were optimized by using rhizomatic material of cultivated Ligusticum chuanxiong.RESULTS The results suggest that the best denaturant gradient range is from 45% to 80%,the optimal electrophoresis time is 160 min.CONCLUSION This method is simple and quick,and layed a foundation for the further study on the analysis of the community of endophytic fungi for Ligusticum chuanxiong." @default.
- W2349780147 created "2016-06-24" @default.
- W2349780147 creator A5009768180 @default.
- W2349780147 date "2013-01-01" @default.
- W2349780147 modified "2023-09-25" @default.
- W2349780147 title "Establishment and optimization of PCR-DGGE technique on endophytic fungi for Ligusticum chuanxiong" @default.
- W2349780147 hasPublicationYear "2013" @default.
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