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- W2349837519 abstract "To express chicken osteoprotegerin(chOPG)in Pichia pastoris and determine its inhibitive effects on mature osteoclastic bone resorptive function in vitro,the chOPG cDNA encoding for 382 amino acid without signal peptide was subcloned into Pichia pastoris expression vector pPICZα-A.The constructed recombinant plasmid was transformed into yeast host cell X-33 by electroporation.The positive recombinant transformants were selected with PCR from Zeocin+ YPDS plate.Induced by the addition of methanol every 24 hours,mainly owing to heterogeneous glycosylation,the product relative moleculer mass was about 43 000 and 53 000 respectively at a yield of 200 mg per litter of culture.The result of Western-blot indicated that the recombinant protein had specific antigenicity.The chOPG recombinant protein at certain concentration could restrain the activity of mature osteoclasts in vitro,that is to say,the number and area of the bone resorptive lacunae both decreased." @default.
- W2349837519 created "2016-06-24" @default.
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- W2349837519 date "2009-01-01" @default.
- W2349837519 modified "2023-09-27" @default.
- W2349837519 title "Secreting expression of chicken osteoprotegerin (chOPG) in Pichia pastoris and the bioactivity of recombinant protein" @default.
- W2349837519 hasPublicationYear "2009" @default.
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