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- W2349901173 abstract "Objective: To produce the GST PTB domain fusion protein. Methods: The cDNA encoding p62Dok PTB domain was amplified by using PCR method, and cloned into pGEX 4T 3 vector. The expression plasmid was introduced into E.coli strain BL 21. The GST PTB fusion protein accounted for over 25% of the total bacterial protein after IPTG induction, it was purified to homogeneity from the cell lysates via affinity chromatography; and analyzed by SDS PAGE gel.Results: The recombinant GST PTB fusion proteins were prepared with high purity, more than 95%, and high recovery, about 75%. Conclusion: The established method for preparation large quantity recombinant GST PTB fusion proteins would promote the structure function analysis of the PTB domain of p62 Dok protein." @default.
- W2349901173 created "2016-06-24" @default.
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- W2349901173 date "2003-01-01" @default.
- W2349901173 modified "2023-09-25" @default.
- W2349901173 title "Expression and purification of the GST fusion protein of p62Dok PTB domain" @default.
- W2349901173 hasPublicationYear "2003" @default.
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