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- W2349904173 abstract "Objective:To explore the effect on growth of MCF-7 cells by over-expressing nuclear receptor coactivator PNRC(proline-rich nuclear receptor coregulatory protein).Methods:To construct the recombinant plasmid pCMVtaq2c/PNRC,full length PNRC cDNA with Bcl I and Xho I amplified by PCR was digested and inserted into eukaryotic expression plasmid pCMVtaq2c.After identification of restriction endonuclease and sequencing,the recombinant plasmid and empty plasmid was respectively transfected into MCF-7 cells by lipofectAMINE.The stably transfected strain after G418 screening for 4 weeks was identified with RT-PCR,Northern blot and Western blot.The different growth rate and proliferation of MCF-7 cells over-expressing PNRC and empty plasmid transfected were detected by MTT.Results:The eukaryocytic expression plasmid pCMVtaq2c/PNRC was constructed successfully.MCF-7 cell strain stably expressing PNRC was screened and the growth rate and proliferation of MCF-7 cells transfected PNRC was obviously slower than that of control cells.Conclusion:PNRC over-expression could inhibit the growth of MCF-7 cells." @default.
- W2349904173 created "2016-06-24" @default.
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- W2349904173 date "2007-01-01" @default.
- W2349904173 modified "2023-09-27" @default.
- W2349904173 title "Effect on growth of MCF-7 cells by PNRC over-expression" @default.
- W2349904173 hasPublicationYear "2007" @default.
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