Matches in SemOpenAlex for { <https://semopenalex.org/work/W2349931524> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2349931524 abstract "Objective: Cloning manganese superoxide dismutase gene (sodA) to make it express in Lactobacillus bulgaricus (L6032) to enhance its tolerance to oxygen was studied. Moreover, a foundation for developing SOD fermented milk was established. Methods: Using PCR to amplify sodA and cloning it into the Lactic acid bacteria expression vector pMG36e and then transforming this recombinant plasmid pMG36e-sod into L6032 by electroporation were studied; SOD reagent was used to detect its activity and to compare the growing situation and living condition as aerobes in MRS culture medium between L6032 and L6032-SOD. A preliminary inquiry into the effect of anti-oxygen of L6032 with SOD expression was made. Results: Construct- ing the recombinant plasmid pMG36e-sod successfully has been identified with the prospect by means of enzyme digestion and PCR. The result of sequencing showed that the insert gene could encode SOD correctly; After successfully transforming pMG36e-sod into L6032 by electroporation the activity of SOD was about 590.5NU∕mg prot. Furthermore, enhancing L. bulgaricus's tolerance to oxygen has not only made L6032-SOD grow better than L6032 under the same condition, but also improved the living rate of bacteria obviously. Conclusion: sodA has been successfully for expressed in L. bulgaricus to have enhanced its tolerance to oxygen. It was beneficial to develop and apply Lactobacillus. At the same time, this study has laid a foundation for further developing SOD fermented milk." @default.
- W2349931524 created "2016-06-24" @default.
- W2349931524 creator A5046711953 @default.
- W2349931524 date "2005-01-01" @default.
- W2349931524 modified "2023-09-27" @default.
- W2349931524 title "Cloning and Expression of the Manganese Superoxide Dismutase Gene of E. coli in Lactobacillus bulgaricus" @default.
- W2349931524 hasPublicationYear "2005" @default.
- W2349931524 type Work @default.
- W2349931524 sameAs 2349931524 @default.
- W2349931524 citedByCount "0" @default.
- W2349931524 crossrefType "journal-article" @default.
- W2349931524 hasAuthorship W2349931524A5046711953 @default.
- W2349931524 hasConcept C100544194 @default.
- W2349931524 hasConcept C104317684 @default.
- W2349931524 hasConcept C121050878 @default.
- W2349931524 hasConcept C181199279 @default.
- W2349931524 hasConcept C185592680 @default.
- W2349931524 hasConcept C186852380 @default.
- W2349931524 hasConcept C199360897 @default.
- W2349931524 hasConcept C2775838275 @default.
- W2349931524 hasConcept C2780206646 @default.
- W2349931524 hasConcept C31903555 @default.
- W2349931524 hasConcept C41008148 @default.
- W2349931524 hasConcept C523546767 @default.
- W2349931524 hasConcept C54355233 @default.
- W2349931524 hasConcept C547475151 @default.
- W2349931524 hasConcept C55493867 @default.
- W2349931524 hasConcept C86803240 @default.
- W2349931524 hasConcept C89423630 @default.
- W2349931524 hasConceptScore W2349931524C100544194 @default.
- W2349931524 hasConceptScore W2349931524C104317684 @default.
- W2349931524 hasConceptScore W2349931524C121050878 @default.
- W2349931524 hasConceptScore W2349931524C181199279 @default.
- W2349931524 hasConceptScore W2349931524C185592680 @default.
- W2349931524 hasConceptScore W2349931524C186852380 @default.
- W2349931524 hasConceptScore W2349931524C199360897 @default.
- W2349931524 hasConceptScore W2349931524C2775838275 @default.
- W2349931524 hasConceptScore W2349931524C2780206646 @default.
- W2349931524 hasConceptScore W2349931524C31903555 @default.
- W2349931524 hasConceptScore W2349931524C41008148 @default.
- W2349931524 hasConceptScore W2349931524C523546767 @default.
- W2349931524 hasConceptScore W2349931524C54355233 @default.
- W2349931524 hasConceptScore W2349931524C547475151 @default.
- W2349931524 hasConceptScore W2349931524C55493867 @default.
- W2349931524 hasConceptScore W2349931524C86803240 @default.
- W2349931524 hasConceptScore W2349931524C89423630 @default.
- W2349931524 hasLocation W23499315241 @default.
- W2349931524 hasOpenAccess W2349931524 @default.
- W2349931524 hasPrimaryLocation W23499315241 @default.
- W2349931524 hasRelatedWork W1982797899 @default.
- W2349931524 hasRelatedWork W2099450429 @default.
- W2349931524 hasRelatedWork W2103316992 @default.
- W2349931524 hasRelatedWork W2109724863 @default.
- W2349931524 hasRelatedWork W2123867459 @default.
- W2349931524 hasRelatedWork W2137947138 @default.
- W2349931524 hasRelatedWork W2347632699 @default.
- W2349931524 hasRelatedWork W2347857588 @default.
- W2349931524 hasRelatedWork W2350173075 @default.
- W2349931524 hasRelatedWork W2350389667 @default.
- W2349931524 hasRelatedWork W2354696218 @default.
- W2349931524 hasRelatedWork W2364461393 @default.
- W2349931524 hasRelatedWork W2383904413 @default.
- W2349931524 hasRelatedWork W2387889411 @default.
- W2349931524 hasRelatedWork W2414891326 @default.
- W2349931524 hasRelatedWork W37474338 @default.
- W2349931524 hasRelatedWork W2854907661 @default.
- W2349931524 hasRelatedWork W2867185285 @default.
- W2349931524 hasRelatedWork W2877443223 @default.
- W2349931524 hasRelatedWork W2956763078 @default.
- W2349931524 isParatext "false" @default.
- W2349931524 isRetracted "false" @default.
- W2349931524 magId "2349931524" @default.
- W2349931524 workType "article" @default.