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- W2349931760 abstract "Objective To observe the changes of cytosol-free calcium concentration in single bladder smooth muscle cell from T2DM rats in order to elucidate the pathogenesis of the damage in the T2DM rats' detrusor. Methods Rat T2DM model was developed and age-matched Wistar rats served as controls. Four and 20 weeks later, the effects of diabetes mellitus on intracellular calcium concentration were measured in single bladder smooth muscle cell in rats using the fluorescent calcium indicator dye Fu-ra-2/AM at rest situation. Results The intracellular calcium concentration in single bladder smooth muscle cell in T2DM rats had no significant difference in the initial stage at rest situation in comparison to control group (289.24 ± 33.80 vs 276.85 ± 57.90). However, at 20th week after onset, the intracellular calcium concentration in single bladder smooth muscle cell (545.94 ± 79.28) was significantly increased in T2DM rats as compared with that in control group (275.66 ± 108.94). Conclusion Intracellular calcium overload occurred later than the dysbolism. Maybe intracellular calcium overload is an important factor in the progression of diabetic cystopathy." @default.
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- W2349931760 date "2006-01-01" @default.
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- W2349931760 title "Changes of cytosol-free calcium concentration in single bladder smooth muscle cell from T2DM rats" @default.
- W2349931760 hasPublicationYear "2006" @default.
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