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- W2349952665 abstract "Objective To construct and purify the flexible peptide linked quadrivalent Aβ15 Adenovirus vaccine for preventing and therapy of the Alzheimer disease(AD).Methods The IgG к light chain fused flexible peptide linked quadrivalent Aβ15 sequence was acquired by restricted enzyme digestion.And it was cloned to the shuttle plasmid pAdTrack-CMV.The homologous recombination between the recombinant vector and the adenovirus bone vector pAdeasy-1 in the Ecoli BJ5183 resulted to the formation of recombinant adenovirus vector pAd-4×Aβ15.The infecting recombinant virus particles Ad-4×Aβ15 was produced after the recombinant adenovirus vector had been transfected to the human embry kidney 293 cells.Western blotting detected the secreted expression of 4×Aβ15 protein in the supernatant.Ad-4×Aβ15 was purified by CsCl2 density gradient ultracentrifuge.Results The sequence results of pAdTrack-4×Aβ15 were consistent with expectancy.After homologous recombination and packaging with 293 cells,the recombinant Ad-4×Aβ15 adenovirus was obtained.Western blotting suggested that the 4×Aβ15 protein was expressed secretly in the supernatant.After CsCl2 density gradient ultracentrifuge,the particles of Ad-4×Aβ15 adenovirus were about 4.6×1012 VP/mL.Conclusions The Ad-4×Aβ15 was constructed successfully.It is confirmed that the secreted interest proteins were expressed in the infected cells.After CsCl2 density gradient ultracentrifuge,the high titre Ad-4×Aβ15 adenovirus were obtained,which is important for AD experimental therapy." @default.
- W2349952665 created "2016-06-24" @default.
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- W2349952665 date "2010-01-01" @default.
- W2349952665 modified "2023-09-25" @default.
- W2349952665 title "Construction and purification of flexible peptide linked quadrivalent Aβ_(15) adenovirus" @default.
- W2349952665 hasPublicationYear "2010" @default.
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