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• W2349960169 abstract "Objective:To investigate effect of valproic acid(VPA) with different dose and treated time on differentiation of neural stem cells(NSCs).Methods:Different dose of VPA(0.1 mmol/L,0.3 mmol/L,0.5 mmol/L,0.75 mmol/L and 1.0mmol/L)were used to en-hance the differentiation of NSCs isolated and cultured in serum-free medium from the SD rats pregnant for 14 days.The NSCs in neural basal medium is the control group.Differentiated cells at 3rd day,7th day,10th day and 14th day were stained by DAPI,β-tubllin III and GFAP.And the ratio of differentiated cells was analysed.Results:The differentiated ratio of neurons at 3rd day had no disparity.At 7th day、10th day and 14th day the differentiated ratio of neurons in VPA groups was higher than that in control group.The differentiated ratio of neurons in 0.75 mmol/L VPA group was 82.15±0.93% at 10th day.But the differentiated ratio of neurons at 10th day and 14th day had no disparity.At 3 to 10 days the differentiated ratio of neurons in each group increased apparently,but at 14th day the differenti-ated ratio in each group didn't increased.Conclusion:0.75 mmol/L VPA at 10th day produces the best effect on promoting the NSCs dif-ferentiated into neurons." @default.
• W2349960169 created "2016-06-24" @default.
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• W2349960169 date "2011-01-01" @default.
• W2349960169 modified "2023-09-25" @default.
• W2349960169 title "The Dose and Time Effect of Valproic Acid on Differentiation of Neural Stem Cells" @default.
• W2349960169 hasPublicationYear "2011" @default.
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