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- W2349969646 abstract "Objective To establish a multiplex PCR assay for the identification of S. aureus from the Staphylococcus isolates and for the simultaneous detection of clinically relevant antibiotic-resistant genes in these isolates at the species level.Methods Simultaneous amplification of the 310bp,456bp,and 651bp regions of the mecA,ileS and femB genes were respectively performed by using the rapidly extracted DNA from single Staphylococcus colony in single tube.The mecA and ileS fragments amplified by PCR were compared to those in antibiotic susceptibility tests of methicillin and mupirocin,respectively,and the resistance phenotypes were determined.Results The PCR detection of femB fragment allowed the specific identification of S.aureus from the Staphylococcus isolates.The PCR detection of mecA fragment showed a consistent result with that of methicillin-resistant test and indicated the mecA gene may be used as a useful molecular marker for diagnosis of methicillin-resistant Staphylococcus (MRS).The Staphylococcus isolates with ileS gene appeared to be mupirocin-resistance.Conclusion The multiplex PCR offers a rapid,sensitive test to identify S.aureus from the Staphylococcus isolates.MRSA and mupirocin-resistant genes in clinical isolates can be detected by the PCR assay simultaneously." @default.
- W2349969646 created "2016-06-24" @default.
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- W2349969646 date "2004-01-01" @default.
- W2349969646 modified "2023-09-27" @default.
- W2349969646 title "Identification of Staphylococcus aureus and detection of its multiple-resistant genes by multiplex PCR" @default.
- W2349969646 hasPublicationYear "2004" @default.
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