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- W2349978874 abstract "Objective: To study the influences of dibutyl phthalate(DBP) on the estrous cycle and endocrine of puberty female rats.Methods: 21-day-old female SD rats were randomly divided into 250 mg/kg,500 mg/kg and 1 000 mg/kg experimental groups and one control group(corn oil),the rats were administrated with DBP by gavage for 8 weeks.Female rats were sacrificed,during the proestrus,and the blood was collected,and the serum levels of progesterone(P) were examined by radioimmunoassay.The mRNA levels of cholesterol side-chain cleavage enzyme(P450scc) of ovaries were detected by real-time fluorescent quantitative polymerase chain reaction(RTPCR).Results: There were statistically significant differences of serum progesterone levels among treatment and control groups(F=4.306,P=0.014),the serum progesterone level in 1 000 mg/kg does group was lower than that in the control group(P0.05),while there was no significant difference of serum progesterone between 250 mg/kg or 500 mg/kg groups and the control group(P0.05).The expression levels of P450scc mRNA in ovarian were significant different in treatment and the control groups(H=15.591,P0.01),compared with the control group,the expression levels of P450scc mRNA in three treatment groups were lower(P0.01),but there were not significant differences in expression levels of P450scc mRNA among three treatment groups(P0.05).Conclusion: DBP exposure have reproductive toxicity to female rats." @default.
- W2349978874 created "2016-06-24" @default.
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- W2349978874 date "2012-01-01" @default.
- W2349978874 modified "2023-09-23" @default.
- W2349978874 title "Influence of dibutyl phthalate on the gene expression of P450scc of ovary in pubertal female rats" @default.
- W2349978874 hasPublicationYear "2012" @default.
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