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- W2350008868 abstract "The coelomocytes of sea cucumber,Apostichopus japonicas,was cultured in vitro using three different culture mediums,namely CCM,HSM,L-15N,and the MTT reduction method was employed to detect the cells viability in these mediums.The coelomocytes viability of sea cucumber decreased significantly at day 3 and day 6 after being cultured in vitro with CCM and HSM respectively.However,L-15N medium could keep coelomocytes alive stably in vitro for at least one week,without significant viability loss.Extracellular products(ECP) of pathogenic bacteria,Vibrio splendidus,were obtained and shows strong cytotoxicity toward coelomocytes of sea cucumber incubated in vitro,and the protein concentration of ECP that caused the death of 50% of the cell population(IC50) was 27.6 μg/mL." @default.
- W2350008868 created "2016-06-24" @default.
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- W2350008868 date "2010-01-01" @default.
- W2350008868 modified "2023-09-22" @default.
- W2350008868 title "Culture of Coeleomocytes of Sea Cucumber,Apostichopus japonicas in Vitro and the Cytotoxicity Effect of Extracellular Products of Vibrio splendidus to the Coelomocytes" @default.
- W2350008868 hasPublicationYear "2010" @default.
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