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- W2350100308 abstract "Objective:To establish a grastrointestinal stromal tumor(GIST) cell line and to determine its drug sensitivity to STI571.Methods:GIST cells were separated from fresh GIST specimens through dispase digestion and then were immotalized by ectopic expression of telomerase reverse transcriptase(hTERT) and simian virus 40 large T(SV40LT) antigen.The effect of STI571 on this cell line was detected by MTT dye.Results:The cells showed fusiform shape and formed monolayer in vitro culture;they expressed c-kit,hTERT,and SV40LT antigens.STI571 significantly inhibited the proliferation of the cells.Conclusion:The cells have the properties of GIST and can be used as an important tool for studying the carcinogenesis and resistant mechanism of GIST." @default.
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- W2350100308 date "2010-01-01" @default.
- W2350100308 modified "2023-09-24" @default.
- W2350100308 title "Establishment and Immortalization of Human Gastrointestinal Stromal Tumor Cells" @default.
- W2350100308 hasPublicationYear "2010" @default.
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