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- W2350118069 abstract "The experiment was conducted to achieve expression of PIL 6 gene in E.coli ,to separate fusion protein which contains GST and PIL 6,to test it's bioactivity and prepare rabbit anti PIL6 serum.Methods:The PIL 6 cDNA fragment was inserted into pGEX 1λT and the recombinant plasmid was identified by BamH I and Pst I digest.The expression product was assayed by RT PCR and SDS PAGE.The fusion protein was recovered from polyacrylamide gel and it's bioactivity was tested by stimulating the proliferation of lymphoblast cells from the spleen of mice.Rabbit was immunized with the fusion protein.Result:The recombinant plasmid expressing PIL 6 in a high level was obtained,a special DNA band of 740bp was observed in the electophoresis result of RT PCR product,a new protein band was found with molecular weight of about 49kD in SDS PAGE.The PIL 6 fusion protein significantly promoted the proliferation of lymphoblast cells from mice,in comparison with the control,and the titer of rabbit anti PIL 6 serum was 1∶128 000." @default.
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- W2350118069 date "2002-01-01" @default.
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- W2350118069 title "Prokaryotic Expression of Porcine Interleukin-6 Gene And The Study It's Bioactivity" @default.
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