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- W2350118709 abstract "Objective:To investigate the inhibitory effect of baicalin on the fluorouracil(Fu)resistant hepatocarcinoma cell(HCC)BEL-7402/5-Fu and its possible mechanism.Methods:Hepatocarcinoma cell line BEL-7402 and Fu-resistant hepatocarcinoma cell line BEL-7402/5-Fu were cultured in vitro.The inhibitory effect of baicalin on the BEL-7402/5-Fu cells was assessed by MTT assay;the intracellular rhodamine fluorescence intensity was observed by flow cytometry;the expression of MDR1 gene was detected by RT-PCR;and the expression of protein P-glycoprotein(P-gp)was analyzed by Western blotting assay.Adhesion assay was conducted using Matrigel model.Expression of beta 1-integrin and E-CD protein was detected by immunofluorescence technique.Results:Baicalin inhibited the proliferation of both BEL-7402 and BEL-7402/5-Fu cells,with IC50 of baicalin being 34.2 mg/L and 36.6 mg/L,respectively.Baicalin(5 mg/L and 10 mg/L)partially reversed the resistance of BEL-7402/5-Fu to Fu,with the reversal folds being 28.6 and 46.7,respectively.Baicalin(5 mg/L and 10 mg/L)increased the sensitivity of BEL-7402 cells to Fu,with the sensitivity-enhancing folds being 1.4 and 2.1,respectively.Baicalin also increased the concentration of rhodamine and expression of integrin β1,inhibited the expression of MDR1 gene and P-gp,E-CD protein,and reduced the adhesion capacity,with the effect of 10 mg/L baicalin significantly effective than that of 5 mg/L baicalin(all P0.05).Conclusion:Baicalin can inhibit the proliferation of BEL-7402/5-Fu in vitro,and partially reverse the resistance to Fu,which is attributable to the increased accumulation of intracellular drug concentration,inhibited expression of MDR1 gene." @default.
- W2350118709 created "2016-06-24" @default.
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- W2350118709 date "2008-01-01" @default.
- W2350118709 modified "2023-09-23" @default.
- W2350118709 title "Inhibitory effect of baicalin on fluorouracil resistant HCC cell line BEL-7402/5-Fu and its possible mechanism" @default.
- W2350118709 hasPublicationYear "2008" @default.
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