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- W2350120849 abstract "Amplified apoA-I CDS(region)from pMD18-T pl as mid and apoA-I promoter(702bp)DNA segment from human genomic DNA were cloned in pEGFP-N1 plasmid to generate a series of fusion protein expression plasmids. The expression of the fusion proteins are under the regulation of apoA-I promo ter. HepG2 cells were transfected with these fusion protein expression plasmids, and a series of stable fusion protein expression cell clones were screened by e nhanced green fluorescent protein(EGFP)marker. With RT-PCR,fluorescent micro scope,immunity fluorescent and other methods, one stable fusion protein express ion clone was identified. Insulin and glucose regulate the fusion protein expres sion. The results demonstrate that human apoA-I promoter can regulate gene expr ession of human liver cell in response to glucose and insulin in a similar way a s endogenous apoA-I,and the liver cell pattern of human apoA-I gene regulatio n and secretory expression has been established primarily." @default.
- W2350120849 created "2016-06-24" @default.
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- W2350120849 date "2005-01-01" @default.
- W2350120849 modified "2023-09-23" @default.
- W2350120849 title "The Cell Pattern Construction of Human apoA-I Gene Regulation and Secretory Expression" @default.
- W2350120849 hasPublicationYear "2005" @default.
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