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- W2350168735 abstract "Purpose:The aim was to identify the characteristic of engineering cell of human erythropoietin.Methods:The recombinant plasmid pCDB constructed with EPOcDNA and Marked plasmid pSV-dhfr was cotransfected into CHO-dhfr~- cell.The cloneof the highest expression EPO were selected and identified through methotrexate amplification and subclone.Results:The results of identification show that F10-6 cell line have not been contaminated by mycoplasma,bacteria,fungi,and virus.It also have no the form of tumor in nude mice.The chromosomal number of F10-6 cell line is normal.It was bequeathed for fifty passages and resuscitated after freezing three times.The expression level of EPO is stable.Conclusion:F10-6 engineering cell can be used for the production of industrialization." @default.
- W2350168735 created "2016-06-24" @default.
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- W2350168735 date "2004-01-01" @default.
- W2350168735 modified "2023-09-27" @default.
- W2350168735 title "The Analysis and Identify of Characteristics for the Engineering Cell Lines of Erythropoietin" @default.
- W2350168735 hasPublicationYear "2004" @default.
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