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- W2350182156 abstract "The addition of cryoprotective agents(CPAs) to cells is critical to protect cells from freezing damage during cryopreservation.In this study,volume change of cells and the concentration change of CPA inside cells were simulated using the Kedem-Katchalsky model in order to gain the knowledge of volume damage and osmotic injury to cells and optimize protocols of CPA loading.Results showed that,for CPA at the low concentration,the volume damage and osmotic injury to cells are weak during one-step addition and therefore one-step addition can be used.For CPA at high concentration,damage to cells is reduced significantly with addition by both stepwise and continuously compared with one-step addition.They can smaller the damages of both volume change and permeability during cryopreservative agent loading.In addition,four-step addition is better than others in the stepwise addition patterns.For the continuous CPA addition,linear and S-mould CPA addition patterns are preferred." @default.
- W2350182156 created "2016-06-24" @default.
- W2350182156 creator A5042230662 @default.
- W2350182156 date "2008-01-01" @default.
- W2350182156 modified "2023-09-28" @default.
- W2350182156 title "Modeling and optimization of process of cryoprotective agent addition" @default.
- W2350182156 hasPublicationYear "2008" @default.
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