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- W2350182655 abstract "Microsatellite DNA has been shown to be highly polymorphic and co dominant and therefore quite useful as a good genetic marker.However,the wide utilization of microsatellite marker is hindered by the difficulty of obtaining microsatellite loci from interesting species.Recently,many methods have been developed for the isolation of microsatellite loci.Among these methods,database searching is the most convenient method for obtaining new microsatellite loci,such as acquiring primers from published literatures.In recent years,with the increase of EST database,SSRs explored from dbEST have been used in many species.In addition,the conservation of repeat sequence and flanking regions across taxa makes it possible that microsatellite primers from one species can be used to detect polymorphism at homologous sites in related species.The last method is to exploit microsatellite loci from genomic DNA,including the traditional method of genomic library and the novel approach of enriched library such as magnetic bead hybridization and nylon membrane hybridization." @default.
- W2350182655 created "2016-06-24" @default.
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- W2350182655 date "2005-01-01" @default.
- W2350182655 modified "2023-09-23" @default.
- W2350182655 title "The Methods of Isolating Microsatellite Loci" @default.
- W2350182655 hasPublicationYear "2005" @default.
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