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- W2350212116 abstract "Objective To analyze the variability and phenotype of envelope glycoprotein(env) of human immunodeficiency virus prevalent in Heilongjiang province. Methods Full-length env gene cloning from PBMC of a HIV-1 positive individual in Heilongjiang province, China was performed with conserved region primers. The amplified PCR products were cloned into a plasmid vector and sequenced. Phylogenetic tree analysis was done upon full length env amino acid sequence. Subsequently, a HIV-1 pseudotyped virus with the envelope protein was constructed and the infectivity was examined with U87 cell lines expressing CD4 with either CCR5 or CXCR4. ResultsTwo functional env clones named CHNHLJ03009c34(GenBank Accession No: AY905493) and CHNHLJ03009c33 were obtained. The homology between CHNHLJ03009c34 and a HIV-1 B′ subtype RL42 strain from Yunnan province is 91.52% through comparing and analyzing full length env amino acid sequence from domestic and abroad isolates. Phylogenetic analysis indicated that CHNHLJ03009c34 had an intimate molecular relation to strain RL42, while it became an independent arm upon the analysis of C2-V3 region. The secondary structure analysis of the envelope protein showed that the antigenicity and hydropathy of the strain did not have definite difference from RL42. Infectivity examination showed that the pseudovirus could only infect U87.CD4.CCR5 cells indicating that it comes from R5-tropic HIV-1. Conclusion HIV-1 primary isolate envelopes CHNHLJ03009 have been cloned from a HIV-1 positive individual in Heilongjiang province. The strain is R5 Thailand B′ subtype. This is the first analysis of primary isolates in Heilongjiang." @default.
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- W2350212116 date "2005-01-01" @default.
- W2350212116 modified "2023-09-25" @default.
- W2350212116 title "Cloning and analysis of HIV-1 envelope isolated from an infected individual in Heilongjiang province" @default.
- W2350212116 hasPublicationYear "2005" @default.
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