Matches in SemOpenAlex for { <https://semopenalex.org/work/W2350216650> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2350216650 abstract "[Objective] To construct a cDNA library of human embryonic stem(hES) cell for screening,cloning and studying new genes.[Methods] Thomson-derived human embryonic stem cells were cultured and isolated.Total RNA was extracted from hES cell and mRNA was purified using mRNA oligo(dT) magnetic particle purification technique.The first and second strand cDNA was synthesized by reverse transcriptase and DNA polymerase I respectively.The ds-cDNA termini was blunted with pfu DNA polymerase.The blunted cDNAs were added EcoRⅠadaptor and then digested by XhoⅠ.The cDNA fragments longer than 0.5 kb were collected by Sepharose CL2B chromatography and ligated to Uni-ZAP XR expression vector.The ligated products were packaged in vitro and the lambda phage particles infected the host strains XL1-BlueMRF' to produce an unamplified library.The library was amplified and tittered.The size of cDNA fragment was evaluated by EcoRⅠand XhoⅠ restriction endonuclease digestion.[Results] The efficiency of the primary library was 1.2 ×106 recombinant and the amplified library was 1.0 ×109 pfu /mL.The percentage of positive recombinant clones was 98.5%.The length of the inserted cDNA fragment was over 1 kb.[Conclusion] The quality of the constructed hES cDNA library is excellent and helpful to screen new genes." @default.
- W2350216650 created "2016-06-24" @default.
- W2350216650 creator A5017054782 @default.
- W2350216650 creator A5075338340 @default.
- W2350216650 date "2007-01-01" @default.
- W2350216650 modified "2023-10-01" @default.
- W2350216650 title "Construction and characterization of a cDNA library from human embryonic stem cells" @default.
- W2350216650 hasPublicationYear "2007" @default.
- W2350216650 type Work @default.
- W2350216650 sameAs 2350216650 @default.
- W2350216650 citedByCount "0" @default.
- W2350216650 crossrefType "journal-article" @default.
- W2350216650 hasAuthorship W2350216650A5017054782 @default.
- W2350216650 hasAuthorship W2350216650A5075338340 @default.
- W2350216650 hasConcept C104317684 @default.
- W2350216650 hasConcept C105580179 @default.
- W2350216650 hasConcept C121050878 @default.
- W2350216650 hasConcept C128319531 @default.
- W2350216650 hasConcept C13575015 @default.
- W2350216650 hasConcept C1436000 @default.
- W2350216650 hasConcept C153911025 @default.
- W2350216650 hasConcept C156719811 @default.
- W2350216650 hasConcept C167625842 @default.
- W2350216650 hasConcept C187882448 @default.
- W2350216650 hasConcept C189482833 @default.
- W2350216650 hasConcept C19924922 @default.
- W2350216650 hasConcept C199360897 @default.
- W2350216650 hasConcept C40767141 @default.
- W2350216650 hasConcept C41008148 @default.
- W2350216650 hasConcept C42062724 @default.
- W2350216650 hasConcept C50724178 @default.
- W2350216650 hasConcept C54355233 @default.
- W2350216650 hasConcept C552990157 @default.
- W2350216650 hasConcept C67705224 @default.
- W2350216650 hasConcept C86803240 @default.
- W2350216650 hasConceptScore W2350216650C104317684 @default.
- W2350216650 hasConceptScore W2350216650C105580179 @default.
- W2350216650 hasConceptScore W2350216650C121050878 @default.
- W2350216650 hasConceptScore W2350216650C128319531 @default.
- W2350216650 hasConceptScore W2350216650C13575015 @default.
- W2350216650 hasConceptScore W2350216650C1436000 @default.
- W2350216650 hasConceptScore W2350216650C153911025 @default.
- W2350216650 hasConceptScore W2350216650C156719811 @default.
- W2350216650 hasConceptScore W2350216650C167625842 @default.
- W2350216650 hasConceptScore W2350216650C187882448 @default.
- W2350216650 hasConceptScore W2350216650C189482833 @default.
- W2350216650 hasConceptScore W2350216650C19924922 @default.
- W2350216650 hasConceptScore W2350216650C199360897 @default.
- W2350216650 hasConceptScore W2350216650C40767141 @default.
- W2350216650 hasConceptScore W2350216650C41008148 @default.
- W2350216650 hasConceptScore W2350216650C42062724 @default.
- W2350216650 hasConceptScore W2350216650C50724178 @default.
- W2350216650 hasConceptScore W2350216650C54355233 @default.
- W2350216650 hasConceptScore W2350216650C552990157 @default.
- W2350216650 hasConceptScore W2350216650C67705224 @default.
- W2350216650 hasConceptScore W2350216650C86803240 @default.
- W2350216650 hasLocation W23502166501 @default.
- W2350216650 hasOpenAccess W2350216650 @default.
- W2350216650 hasPrimaryLocation W23502166501 @default.
- W2350216650 hasRelatedWork W1563273345 @default.
- W2350216650 hasRelatedWork W2235277374 @default.
- W2350216650 hasRelatedWork W2350255721 @default.
- W2350216650 hasRelatedWork W2354964443 @default.
- W2350216650 hasRelatedWork W2359741505 @default.
- W2350216650 hasRelatedWork W2361478854 @default.
- W2350216650 hasRelatedWork W2363891995 @default.
- W2350216650 hasRelatedWork W2372842158 @default.
- W2350216650 hasRelatedWork W2375797727 @default.
- W2350216650 hasRelatedWork W2377578514 @default.
- W2350216650 hasRelatedWork W2378589352 @default.
- W2350216650 hasRelatedWork W2382449331 @default.
- W2350216650 hasRelatedWork W2389793980 @default.
- W2350216650 hasRelatedWork W2400910423 @default.
- W2350216650 hasRelatedWork W2425295881 @default.
- W2350216650 hasRelatedWork W2483381690 @default.
- W2350216650 hasRelatedWork W3024293617 @default.
- W2350216650 hasRelatedWork W3032439747 @default.
- W2350216650 hasRelatedWork W946294408 @default.
- W2350216650 hasRelatedWork W3029008283 @default.
- W2350216650 isParatext "false" @default.
- W2350216650 isRetracted "false" @default.
- W2350216650 magId "2350216650" @default.
- W2350216650 workType "article" @default.