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- W2350230556 abstract "Species of sea cucumber are morphologically and anatomically distinguishable with each other;however such an easiness is not available for deeply processed sea cucumbers.In this study,dot hybridization method was developed in order to indentify 4 sea cucumber species(Apostichopus japonicus,Cucumaria frondosa,Thelenota ananas,and Parastichopus californicus).Species specific probes were designed according to a 692 bp fragment of mitochondrial cytochrome Ⅰ gene(COⅠ) of the 4 species with Primer premier 5.0.The results showed that the probes hybridize 4 sea cucumbers,respectively,with a high specificity.The sensitivity reached 100 pg genome DNA.Dot hybridization was proved as a convenient and specific technique of identifying sea cucumber species and tracing their sources." @default.
- W2350230556 created "2016-06-24" @default.
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- W2350230556 date "2012-01-01" @default.
- W2350230556 modified "2023-09-25" @default.
- W2350230556 title "Identification of 4 Sea Cucumber Species with Dot Hybridization and Species-Specific Probes" @default.
- W2350230556 hasPublicationYear "2012" @default.
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