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- W2350298360 abstract "To explore the fusion protein of hepatic regeneration factor analog from shark liver(SHRF) and to study its effects on SMMC7721.SHRF analog's cDNA was inserted in the downstream of TrxA in a pET32a expression vector.Recombinant TrxA-SHRF analog fusion protein was expressed under o.2 mmol/L/ IPTG induction in BL21(DE3).After having been purified with His Tag affinity chromatography,TrxA-SHRF analog was cleaved with FXa.Then it purified the SHRSF analog by FPLC Resource(Q.) Its activity on human hepatoma cell lines SMMC7721 was studied by MTT assay.Results: the fusion protein of TrxA-SHRF analog was expressed as a soluble form in bacterial cytosol and the quantity of the fusion protein was above 40% of the total soluble protein of BL21.The molecular weight of the fusion protein was 30 KD.After having been digested by FXa,the recombinant protein of SHRSF analog was detected by SDS-PAGE.The activity analyzed with MTT showed that the purified product could stimulate the proliferation of SMMC7721 cell under the concentration of 50μg/ml,but would inhibit the cells' proliferation up to 200μg/ml." @default.
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- W2350298360 date "2006-01-01" @default.
- W2350298360 modified "2023-09-23" @default.
- W2350298360 title "Cloning,Expression and Purification of the Analog of Hepatic Regeneration Factor from Shark Liver" @default.
- W2350298360 hasPublicationYear "2006" @default.
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