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- W2350313441 abstract "Objective: An indirect competitive light initiated homogeneous time-resolved fluoroimmunoassay ( AlphaLISA) was established by using anti-DON polyclonal antibody and coating antigen DON-BSA for detection of deoxyhivalenol in cereal.Method: DON-BSA was coated on chenilum inescent particles,the AlphaLISA kit also contained sensitizer particles coated with strep tavidin.The optinal test conditions and analytical performance of the method were studied.Results: Working concentration range from 0.007 to 100ng /mL and the sensitivity for detection was 0.007ng /mL.The intra-and inter-assay CV of the assay were both below 5%.The recoveries of determination for deoxynivalenol spiked in corn,wheat,beer were 84%～ 110%、67%～ 100%、74%～ 100% . Conclusions: This AlphaLISA method is a good method with high sensitivity for quantitative analyzing DON in cereal." @default.
- W2350313441 created "2016-06-24" @default.
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- W2350313441 date "2010-01-01" @default.
- W2350313441 modified "2023-09-24" @default.
- W2350313441 title "Quantitative determination of deoxynivalenol using light initiated chemiluminescence assay" @default.
- W2350313441 hasPublicationYear "2010" @default.
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