Matches in SemOpenAlex for { <https://semopenalex.org/work/W2350322034> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W2350322034 endingPage "1883" @default.
- W2350322034 startingPage "1880" @default.
- W2350322034 abstract "OBJECTIVE To optimize the isolation and purification conditions for Hap(s) protein of non-typeable Haemophilus influenzae. METHODS Hap(s) protein was purified by ammonium sulfate precipitation, dialysis desalting and Hitrap weak cation exchange columns of CM Sepharose Fast Flow. The condition of the elution was optimized for pH and ionic strength, the absorbance at 280 nm of the elution samples were detected, and the targeted protein band in the collected samples was observed by SDS-PAGE electrophoresis. RESULTS The Hitrap ion exchange column was eluted with buffer 1, which resulted in a baseline distribution of absorbance at 280 nm. Buffer 2 elution of the column resulted in the presence of peak absorbance with trails, which was identified to be constituted by some low molecular weight bands by subsequent SDS-PAGE. In serial column elution with buffer 3 with different ionic strength, a peak absorbance was observed with the ionic strength of 100 mmol/L NaCl, and SDS-PAGE confirmed that the peak was generated by the target protein. No obvious peaks or bands in SDS-PAGE occurred with the other ionic strengths. CONCLUSION The pH of the buffer only affect the elution of the irrelevant proteins rather than the Hap(s) protein, and elution with the buffer containing 100 mmol/L NaCl can be optimal for eluting the Hap(s) protein." @default.
- W2350322034 created "2016-06-24" @default.
- W2350322034 creator A5013758739 @default.
- W2350322034 creator A5025417188 @default.
- W2350322034 creator A5039084645 @default.
- W2350322034 creator A5044342356 @default.
- W2350322034 creator A5045046511 @default.
- W2350322034 creator A5045202428 @default.
- W2350322034 creator A5066220678 @default.
- W2350322034 date "2007-12-01" @default.
- W2350322034 modified "2023-09-23" @default.
- W2350322034 title "[Optimized isolation and purification of non-typeable Haemophilus influenzae Haps protein]." @default.
- W2350322034 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18159010" @default.
- W2350322034 hasPublicationYear "2007" @default.
- W2350322034 type Work @default.
- W2350322034 sameAs 2350322034 @default.
- W2350322034 citedByCount "0" @default.
- W2350322034 crossrefType "journal-article" @default.
- W2350322034 hasAuthorship W2350322034A5013758739 @default.
- W2350322034 hasAuthorship W2350322034A5025417188 @default.
- W2350322034 hasAuthorship W2350322034A5039084645 @default.
- W2350322034 hasAuthorship W2350322034A5044342356 @default.
- W2350322034 hasAuthorship W2350322034A5045046511 @default.
- W2350322034 hasAuthorship W2350322034A5045202428 @default.
- W2350322034 hasAuthorship W2350322034A5066220678 @default.
- W2350322034 hasConcept C113196181 @default.
- W2350322034 hasConcept C117760992 @default.
- W2350322034 hasConcept C145148216 @default.
- W2350322034 hasConcept C147789679 @default.
- W2350322034 hasConcept C178790620 @default.
- W2350322034 hasConcept C184651966 @default.
- W2350322034 hasConcept C185592680 @default.
- W2350322034 hasConcept C187428577 @default.
- W2350322034 hasConcept C43617362 @default.
- W2350322034 hasConcept C73826308 @default.
- W2350322034 hasConcept C98015330 @default.
- W2350322034 hasConceptScore W2350322034C113196181 @default.
- W2350322034 hasConceptScore W2350322034C117760992 @default.
- W2350322034 hasConceptScore W2350322034C145148216 @default.
- W2350322034 hasConceptScore W2350322034C147789679 @default.
- W2350322034 hasConceptScore W2350322034C178790620 @default.
- W2350322034 hasConceptScore W2350322034C184651966 @default.
- W2350322034 hasConceptScore W2350322034C185592680 @default.
- W2350322034 hasConceptScore W2350322034C187428577 @default.
- W2350322034 hasConceptScore W2350322034C43617362 @default.
- W2350322034 hasConceptScore W2350322034C73826308 @default.
- W2350322034 hasConceptScore W2350322034C98015330 @default.
- W2350322034 hasIssue "12" @default.
- W2350322034 hasLocation W23503220341 @default.
- W2350322034 hasLocation W23503220342 @default.
- W2350322034 hasOpenAccess W2350322034 @default.
- W2350322034 hasPrimaryLocation W23503220341 @default.
- W2350322034 hasRelatedWork W1530363634 @default.
- W2350322034 hasRelatedWork W1972290680 @default.
- W2350322034 hasRelatedWork W2006168157 @default.
- W2350322034 hasRelatedWork W2006950880 @default.
- W2350322034 hasRelatedWork W2010618824 @default.
- W2350322034 hasRelatedWork W2077901942 @default.
- W2350322034 hasRelatedWork W223786966 @default.
- W2350322034 hasRelatedWork W2246219870 @default.
- W2350322034 hasRelatedWork W2349296799 @default.
- W2350322034 hasRelatedWork W2367825925 @default.
- W2350322034 hasRelatedWork W2385275802 @default.
- W2350322034 hasRelatedWork W2389981952 @default.
- W2350322034 hasRelatedWork W2406958334 @default.
- W2350322034 hasRelatedWork W2414930005 @default.
- W2350322034 hasRelatedWork W2417128242 @default.
- W2350322034 hasRelatedWork W2435836929 @default.
- W2350322034 hasRelatedWork W2800201229 @default.
- W2350322034 hasRelatedWork W598682154 @default.
- W2350322034 hasRelatedWork W6013431 @default.
- W2350322034 hasRelatedWork W94378845 @default.
- W2350322034 hasVolume "27" @default.
- W2350322034 isParatext "false" @default.
- W2350322034 isRetracted "false" @default.
- W2350322034 magId "2350322034" @default.
- W2350322034 workType "article" @default.