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- W2350356126 abstract "Objective To investigate the effects of arsenic on primary cuitured skin flbroblast induced by iAsⅢ,DMAⅤ,MMAⅢ.Methods Cell number counting,hiazolyl blue(MTT) reduction assay,transmission inverted microscope were performed in primary cultured skin flbroblast and iAsⅢ,DMAⅤ,MMAⅢ were used as tester inorganic arsenic.Results Primary and F11~F13 cultured skin flbroblast with inverted microscope were oberved;With different concentration of iAsⅢ,DMAⅤ,MMAⅢ(0.5~5.0 μmol/L),the skin fibroblasts were markedly stimulated,and a dose-effect relationship were observed.At concentration of 10~15 μmol/L,however,iAsⅢ,DMAⅤ,MMAⅢ induced injury of skin fibroblasts were observed.Toxicity comparison of skin flbroblast MMAⅢiAsⅢ DMAⅤ.At concentration of 0.5~5.0 μmol/L,MMAⅢ induced proliferation of skin fibroblasts was observed,the crest value was between 0 μmol/L and 1 μmol/L.Conclusion The proliferation of the skin flbroblast line is regulated in a double-phase manner,it may play a critical role in the development of skin injuries caused by inorganic arsenics and the mechanism of DNA injury in primary cultured human skin cells need to be studied further." @default.
- W2350356126 created "2016-06-24" @default.
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- W2350356126 date "2010-01-01" @default.
- W2350356126 modified "2023-09-27" @default.
- W2350356126 title "Effects of proliferation on primary cultured human skin flbroblast induced by ingorganic arsenics and metabolites" @default.
- W2350356126 hasPublicationYear "2010" @default.
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