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- W2350367030 abstract "Objective: To seek lycopene's probably antitumor mechanism. Methods: Technique of cell culture in vitro was used to observe the influence of lycopene on apoptosis phenomena and expression of mutant protein p53 in three cell lines: human cervix tumor cell line (Hela),human hepatoma cell line (QGY-7703,Q-3) and human kidney carcinoma cell line (786-O). Results: Three cancer cells were observed by fluorescence microscopy with acridine orange staining,and the typical apoptosis phenomenon was not found. The two cancer cells: Hela and 786-O dyed by Hoechst No. 33258 were also inspected by fluorescence microscope. The typical apoptosis phenomenon could not be found. Three DNAs extracted from three cells treated with lycopene were checked with DNA agarose gel electrophoresis. The DNAs moved like 'ladder' that were the typical phenomenon of cell apoptosis could not be found in experiments at last. The effect of two carotenoids on expressions of mutant protein p53 of Hela and 786-O were measured and not found evident changes,which was contrast with control. Conclusion: Lycopene has growth inhibition effect on the three cancer cell lines in vitro. The inhibition was not related to apoptosis. The effect would not achieve by inhibiting expression of mutant protein p53." @default.
- W2350367030 created "2016-06-24" @default.
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- W2350367030 date "2004-01-01" @default.
- W2350367030 modified "2023-09-24" @default.
- W2350367030 title "The experimental research on the antitumor mechanism of lycopene in vitro" @default.
- W2350367030 hasPublicationYear "2004" @default.
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