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- W2350432866 abstract "Background and purpose: Bisphosphonates are potent inhibitors of osteoclast-mediated bone resorption,and have been widely used as a therapeutic agent for osteoporosis,Paget's disease,and hypercalcemia associated with cancer.Moreover,there is increasing preclinical evidence that bisphosphonates have direct antitumor effects on a variety of human cancer cells.The purpose of our study was to investigate the mechanism of apoptosis of osteosarcoma cells induced by zoledronic acid,a newly developed third-generation bisphosphonate,and its effect on expression of VEGF.Methods:Cell line LM8(osteosarcoma cells) were cultured and treated with zoledronic acid.Cell Counting Kit-8 assay and flow cytometry were used to detect the proliferation inhibition and apoptosis.Hoechst 33258 staining was used to observe the morphology of the cells.The expression rate of activated caspase-3 and VEGF of LM8 cells were determined by flow cytometry and immuniohistochemical staining respectively.Results:Zoledronic acid could significantly inhibit the growth of LM8 cells in a dose-dependent manner.After being treated with 50 μmol/L zoledronic acid for 48 hours,the proliferation of LM8 cells was significantly inhibited,the inhibition rate was(54±3)% and LM8 cells was observed to present typical apoptosis changes.FCM showed that apoptosis rate was(31.7±2.9)% 48 hours after treatment of 50 μmol/L concentrations of zoledronic acid and the expression rate of activated caspase-3 was up-regulated significantly.The inhibition of expression of VEGF was detected by immuniohistochemical staining.Conclusions:The mechanism of zoledronic acid to induce apoptosis of osteosarcoma cell line LM8 is associated with activation of caspase-3,zoledronic acid and down-regulation of VEGF expression of LM8 cells." @default.
- W2350432866 created "2016-06-24" @default.
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- W2350432866 date "2007-01-01" @default.
- W2350432866 modified "2023-09-27" @default.
- W2350432866 title "Effect of zoledronic acid on proliferation,apoptosis and expression of VEGF of osteosarcoma cell line LM8" @default.
- W2350432866 hasPublicationYear "2007" @default.
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