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- W2350443338 abstract "Objective To verify that siRNA are as efficient as shRNA in gene silencing though the comparative analysis of the silencing efficiency of Synaptotagmin with siRNA and shRNA. Method siRNAs were synthesized by T7 RNA polymerase in vitro and annealed double strand oligonucleotides encoding shRNA were subcloned into the BamHI and HindIII ends of linearized vector H1.1. Then the cells were transfected with siRNAs and shRNAs respectively. The silencing efficiency was determined fluoresence assay and western blot assay.Result siRNA and shRNA showed equivalent efficiency in Syt I and IX gene silencing.Conclusion siRNA can be used as a screen tool in gene silencing and it is an inexpensive and convenient method in gene silencing." @default.
- W2350443338 created "2016-06-24" @default.
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- W2350443338 date "2006-01-01" @default.
- W2350443338 modified "2023-09-23" @default.
- W2350443338 title "Equivalent Efficiency of Synaptotagmin Gene Silencing with siRNA and shRNA" @default.
- W2350443338 hasPublicationYear "2006" @default.
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