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- W2350456792 abstract "Capsular polysaccharide export protein(CPEP)gene of Haemophilus parasuis SC-1 was amplified by PCR and then was cloned into the pMD-19Tvector.After being certified by sequence and digestion with BamHⅠand XhoⅠrespectively,the gene were cloned into the pET-32a(+)vector.The recombinant vector was transformed into BL21(DE3)and then induced by IPTG. The product of expression was studied by SDS-PAGE and Western blot,and then was purified. We further evaluated the immune responses and protective efficacy of purified CPEP in mice models.The results showed that the recombinant vector pET32a-CPEP was constructed successfully. The SDS-PAGE and the western blot results showed that the purified protein was 35kDa and the recombinant CPEP possessed reactogenicity respectively.The protective capacity of the anti-CPEP antibodies was evaluated by the inoculation of highly virulent strain SC-1.Vaccinated animals had a delayed course of disease and 40%of the animals survived the lethal challenge.The partial pro-tection achieved with the recombinant CPEP supports their potential as candidates to be included in future vaccine formulations against H.parasuis." @default.
- W2350456792 created "2016-06-24" @default.
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- W2350456792 date "2014-01-01" @default.
- W2350456792 modified "2023-09-25" @default.
- W2350456792 title "Expression and Immunogenicity Analysis of Capsular Polysaccharide Export Protein of Haemophilus parasuis SC-1 Isolate" @default.
- W2350456792 hasPublicationYear "2014" @default.
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