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- W2350464322 abstract "In order to construct the genetic transformation system of Eucalyptus grandis × E.urophylla‘GLGU9’,Agrobacterium strain,ex-plant types,concentration of Agrobacterium,concentration of acetosyringone in co-culturation medium and timing of co-culturation were optimized.The results indicated that the stem segments were injected with the Agrobacterium LBA4404 containing the plasmid pCAMBIA3301 harboring RaWUS gene in concentration of 0.3 OD for 20 min,co-cultured on the medium without acetosyringone for 48 h,then washed using sterlized water with 400 mg / L cefotaxime,inoculated in differentiation medium also with 400 mg / L cefotaxime,finally regeneration cultured.The transformation ratio of this system was relatively higher,come to 7.14%,which provided an essential fundamental for improving ‘GLGU9 ’with molecular tools." @default.
- W2350464322 created "2016-06-24" @default.
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- W2350464322 date "2013-01-01" @default.
- W2350464322 modified "2023-09-27" @default.
- W2350464322 title "Construction of genetic transformation system for Eucalyptus grandis × E.urophylla ‘GLGU9’" @default.
- W2350464322 hasPublicationYear "2013" @default.
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