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- W2350506426 abstract "Porous poly(glycidyl mathacrylate-divinylbenzene) [P(GMA-DVB)] microspheres containing epoxy groups were synthesized through suspension polymerization. In order to minimize non-reversible adsorption of proteins, polyethylene glycol (PEG) was coupled onto the microspheres via the reaction with epoxy groups. The reaction process was studied and optimized with respect to the amount of PEG coupling. The optimum temperature was 80℃. Bovine serum albumin (BSA) and trypsin were used as two model proteins to examine adsorption and desorption properties of the modified P(GMA-DVB) microspheres. The modified microspheres had little non-reversible adsorption for BSA and trypsin. The mass recovery rate and activity recovery rate were more than 97%. The adsorption of protein on the modified microspheres was reversible hydrophobic interaction. The modified microspheres can be used for hydrophobic interaction chromatography of proteins." @default.
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- W2350506426 date "2006-12-20" @default.
- W2350506426 modified "2023-09-28" @default.
- W2350506426 title "Modification of Poly(Glycidyl Methacrylate-Divinylbenzene) Porous Microspheres with Polyethylene Glycol and Their Use as HIC Medium for Protein Adsorption" @default.
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