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- W2350523613 abstract "The genetic engineering expression vector pGEX-2T-ri was constructed by the insertion of ribonuclease inhibitor (RI) DNA (1.5 kb) that was directly PCR-amplified from the cloning vector pT7-ri into the GST fusion expression vector pGEX-2T. The vector pGEX-2T-ri was transformed into competent E.coli BL21. The molecular weight of GST-RI induced by IPTG was about 76 kD, and its expression level was about 20% of total bacterial proteins. The immunoactivity of GST-RI was confirmed by Western-blot. After renaturation, the fusion protein had the bioactivity to inhibit RNase A (150 U/ml). With treatment of thrombin for 16 hours at 24℃, the fusion protein was cleavaged into 50 kD RI and 26 kD GST." @default.
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- W2350523613 date "2004-01-01" @default.
- W2350523613 modified "2023-09-22" @default.
- W2350523613 title "Fusion Expression and Renaturation of Ribonuclease Inhibitor" @default.
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