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- W2350574822 abstract "The important antigen site of S gene(1 916 bp) of the SC-H strain of TGVE and S1 gene(2 367 bp)of the SC-L strain of PEDV was amplified by RT-PCR and cloned into pMDl9-T vector,the recombinant was named pMD19-T-TS and pMD19T-PS1.which was identified by restriction enzyme and sequenced.Then the S and S1 genes were cut from the recombinant plasmid pMD19-T-TS and pMD19-T-PS1,further inserted into the expression vector pVAXD to construct S1/S eukaryotic co-expression recombinant plasmid pVAXD-PS1-TS.After identifled by restriction enzyme and PCR.the recombinant plasmids were tranfected into COS7 cells,the expressions of recombinant plasmids were confirmed by indirect immunofluorscence assay.The results showed that the eukaryotic co-expression plasmids were constructed successfully and the transfected cells displayed specific immunofluorscence.The successful construction of the pVAXD-PS1-TS provides a foundation for further research of the PEDV and TGEV DNA vaccine." @default.
- W2350574822 created "2016-06-24" @default.
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- W2350574822 date "2012-01-01" @default.
- W2350574822 modified "2023-09-24" @default.
- W2350574822 title "Construction and expression of PEDV and TGEV double gene co-expressing plasmid pVAXD-PS1-TS" @default.
- W2350574822 hasPublicationYear "2012" @default.
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